Unambiguously defined spot colors are colors which have distinct names and associated CMYK equivalents. If two spot colors with different CMYK values have the same name, they will be considered ambiguously defined.
An example would be a document which contains objects from various applications:
If the spot colors of these objects have the same name, say “company green”, but different CMYK values, they are ambiguously defined.

A - Company logo: “company green” C 100%, M 0%, Y 100%, K 50%
B - Separator lines: “company green” C 90%, M 0%, Y 90%, K 50%